Methods I Use

Molecular and developmental biology methods: reverse transcription, various PCR and cloning methods, RACE, in situ hybridization
Neuroanatomical methods: cryotome and Vibratome sectioning and immunolabelling
Neuroanatomical methods: backfills of selected nerve tracts, microinjections of single neurons
Histochemistry and immunocytochemistry in conjunction with fluorescence (FM) and confocal laser scanning microscopy (CLSM)
Transmission electron microscopy (TEM)
Scanning electron microscopy (SEM)
Histology and light microscopy (LM)
Scientific illustration: ink/pencil drawing, digital imaging, volume rendering, 3d-reconstruction
Rearing and breeding of onychophorans in laboratory, both representatives of Peripatopsidae and Peripatidae
Peripatopsis sedgwicki (Peripatopsidae) from South Africa
About Onychophora