Methods we use

Molecular and developmental biology methods: reverse transcription, various PCR and cloning methods, RACE, in situ hybridization
in situ hybridization
Phylogenetic analyses using morphological and molecular data sets
Phylogenetic analyses
Electrophysiology (in collaboration with Prof. Paul A. Stevenson)
Neuroanatomical methods: Cryotome and Vibratome sectioning and immunolabelling
Neuroanatomical methods: backfills of selected nerve tracts, microinjections of single neurons
Proteomics: protein detection by native PAGE and SDS-PAGE, identification of proteins by mass spectrometry (MALDI-TOF, MS/MS), de novo sequencing after protein digestion, and transcriptomics (in collaboration with Prof. Annette G. Beck-Sickinger)
Karyotype analyses using Giemsa and fluorescent DNA markers in conjunction with light and confocal microscopy
Karyotype analyses
Transmission electron microscopy (TEM)
Transmission electron microscopy
Scanning electron microscopy (SEM)
Scanning electron microscopy
X-ray micro-computed tomography techniques such as SRµCT, µCT and nanoCT (in collaboration with Dr. Jörg U. Hammel [Synchrotron DESY, Hamburg] and the Department of Diagnostic and Interventional Radiology of the Technical University of Munich)
Nano-computed tomography
Histology and light microscopy (LM)
Histology and light microscopy
Histochemistry and immunocytochemistry in conjunction with fluorescence (FM) and confocal laser scanning microscopy (CLSM)
Confocal laser scanning microscopy
3D reconstruction of morphological structures
3D reconstruction
Scientific illustration: ink/pencil drawing, digital imaging, volume rendering, 3D-reconstruction
Scientific illustration
Rearing and breeding of onychophorans in laboratory, both representatives of Peripatopsidae and Peripatidae
Rearing and breeding of onychophorans